ABlack Mask - He initially appears to be the main villain, but this is really an impostor. The real Black Mask eventually escapes and attempts to rebuild his criminal empire with a series of drug deals. However, Batman captures him after destroying his hidden drug stashes.The Joker: Initially appearing to be a theatrical nuisance who is slowly escalating, he turns out to be the real mastermind behind the bounty on Batman.The Penguin: He is targeting the once powerful Falcone crime family, and is shown threatening Alberto Falcone. He also has two femme fatale enforcers that Batman must fight. He has not yet received the eye injury he has in Arkham City.Anarky: Reimagined as a black bloc style street protestor, he has placed remote bombs in three locations.Mad Hatter: Triggers a hallucinatory "nightmare level" which serves a spiritual successor to the Scarecrow levels in the first game.The Riddler: At this phase in his career, he is a computer hacker calling himself Enigma. He initially appears to be plotting to blackmail prominent citizens, but it is later suggested that he plans to release his extortion data over the web to drive the city into chaos.Harley Quinn: In the main campaign, she has a cameo in her pre-villainous civilian identity. She is featured more prominently in the Multiplayer mode.Calendar Man: He has a small cameo at the beginning.Mr. Freeze: Though not in the retail version, he features prominently in downloadable content.Many of the 8 super assassins serve as bosses, and each represent a certain skill set which makes conventional attacks ineffective:Killer Croc: He provides the first boss fight. His specialty is brute strengthDeathstroke: The veteran mercenary has an arsenal that rivals the size of Batman's, but primarily uses melee weapons here. He specializes in counterattacks.Deadshot: The master sniper is not an actual boss fight, but he does figure prominently in a side mission. He behaves similar to an armored sniper, but he can use ricochets to circumvent cover, and his weapon deals roughly twice the damage as the standard sniper rifle.Electrocutioner: Stun/shock attacks. He is teased as the second supervillain boss battle, but is actually beaten easily due to his overconfidence (on the lower difficulty settings, he is felled by only one punch). After he is defeated, Batman acquires his shock gloves as a weapon.Lady Shiva: As a ninja master, she specializes in fast combo attacks, cycling between three melee combat styles during her bossfight. She is featured more prominently in side-missions, it is implied that she is an advance agent for Ra's al Ghul.Copperhead: Reimagined as a female contortionist, her primary specialty is poison. Firefly: He serves as the penultimate boss, attempting to destroy the Gotham Bridge. His specialty is explosives.Bane: A masked terrorist with more personal reasons for taking down Batman, he is the secondary antagonist of the storyline, and serves as the game's final boss. Out of all the assassins charged with finding and killing Batman, Bane is the only one who appears to be doing it for personal reasons. He has a repertoire of power moves that result in instant death if they connect.
A1. Blackgate prison.2. The upper levels of the Royal Hotel beyond the Overview Bar. The swimming pool, panorama and penthouse are no longer accessible at the game's end.3. The Bridge: beyond the fire door.4. The shopping mall complex in Burnley can't be accessed after the Shiva side-mission is complete.
ABatman, Gordon, Barbara (Oracle), Riddler (Enigma), Harley, Sharp, and Bruce's parents.