When Harry accesses his father's archive you can see the file names Ravencroft, Dr.Connors, Dr.Morbius, and Venom Storage 7U. Ravencroft is the asylum seen later in this film, Dr. Curt Connors is the Lizard from The Amazing Spider-Man (2012), Dr. Michael Morbius is a Spider-Man villain with vampire-like attributes, and Venom is the space-borne virus that brought out the "Jekyll-Hyde" personality split of both Spider-Man and a villain who took the name Venom for his own villain-name, as filmed in Spider-Man 3 (2007).
When Gwen and Harry talk in the Oscorp elevator, Harry talks to her as if he knows that she is friends with Peter even though she never tells this to him and Peter has not previously told him about her.
During the opening chase when Spidey hangs the last container of plutonium from a lamppost with his webbing, Aleksei easily grabs it, breaking the web. When Spidey immobilises him by webbing his hands, he can't break the webbing.
Aunt May: You know, it's so funny I've been trying to clean up around here to be more organized and putting some of Ben's stuff in boxes, and it's so funny, the heavier the box gets the lighter I feel.
Shailene Woodley had been cast to play Mary-Jane Watson, but all of her scenes were removed from the final film. It was decided her character was not essential to this story.
Who are the villains in this film?
Electro is the major villain. Harry Osborn later becomes the Green Goblin. Rhino also appears to confront Spider-Man at the end of the film.
Does J. Jonah Jameson appear in this film?
No, he never appeared on-screen in this series. He was mentioned a number of times and Peter Parker sent him an e-mail to rethink Spider-Man's negative publicity. Jameson responds with an e-mail saying "WRONG".