According to the interview on Shout! Factory's first DVD box set, Lou Albano had stated that he was not interested in portraying Mario and voicing him at first, but was talked into it by his wife.
For his role as "Mario" in the live-action segments, Lou Albano shaved his trademark goatee. Instead of wearing a false handlebar mustache, he opted to grow a real one.
When shown in reruns after cancellation, DIC took out all the song remixes and replaced them with instrumentals of the songs featured in The Adventures of Super Mario Bros. 3 (1990).
In the Robo-Koopa episode. as appears on the 'Mario's Greatest Movie Moments' DVD, the DVD subtitles misspell "Koopa" as "Kooboo" near the beginning of the episode.